Application of Vanadium and Nickel to the Standard Material for Testing Method of Biocompatibility Using Cultured Cells

Emiko NISHIMURA and Yoshimitsu OKAZAKI*

Japanese Industrial Standards Center, Agency of Industrial Science and Technology, Ministry
of International Trade and Industry; Tsukuba-shi 305-0044 Japan
Mechanical Engineering Laboratory, Agency of Industrial Science and Technology, Ministry
of International Trade and Industry; Tsukuba-shi 305-8564 Japan

The development of standard material to judge cell-culture technique and cell-response is requested in cytotoxicity testing methods for biocompatibility of biomaterials. Vanadium or nickel standard solutions used in inductively coupled plasma (ICP) analysis were added separately at appropriate concentrations to Eagle's medium and α-modified Eagle's medium iα-mediumj. Vanadium or nickel particles were independently extracted in ultra-pure water and Eagle's medium. Then, these extracted solutions were added to fresh Eagle's and α-mediums. Murine fibroblast L929 cell and murine osteoblastic MC3T3-E1 cells were respectively cultured with these Eagle's and α-mediums. The effect of the vanadium and nickel ions in the standard solution used in ICP analysis on cell growth were good accordance with that of the extraction using vanadium and nickel particles. Therefore, it was thought that vanadium or nickel standard solutions used in ICP analysis could be applied as a standard material in biocompatibility tests using cultured cells.



[Contents (In Japanese) ] [Contents (In English) ]