Adsorption of Lysozyme on a Novel Cross-linked Poly (vinyl alcohol) Hydrogel

Hiroki DEGAWA*, Yuki IKEDA, Taiichi SHIROGANE, Takeshi FUKUDA, Osamu WATANABE,
Noriko SAITOH, Kohhei TOMARI, Haruyasu TANIGAWA and Kenji UNO

SEED Co., Ltd.; 1-20-8 Yoshino-cho, Omiya-shi 330-0031 Japan

Lysozyme adsorption to cross-linked poly (vinyl alcohol) (c-PVA) hydrogel was studied in vitro. c-PVA hydrogel was prepared from saponified copolymers of vinyl acetate and triallyl isocyanurate by exposure to ultraviolet light. In vitro, c-PVA hydrogel and four types of 2-hydroxyethyl methacrylate (HEMA) hydrogel were used. Hydrogels were individually kept in the 0.5 w/v% lysozyme solution at 37 °C for 24 hours. The lysozyme adsorbed to hydrogels was extracted with 1 w/v% sodium dodecyl sulfonate (SDS) respectively. The quantity of extracted lysozyme was determined using 2,2'-bicinchoninic acid. The adsorbed lysozyme of c-PVA hydrogel was much less than those of four types of HEMA hydrogel. These results suggest that our PVA hydrogel may be promising as a biomaterial like soft contactlens.

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